Characterization - UV-Vis Spectroscopy

UV-Vis Spectroscopy

UV-Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different components, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. It uses light in the visible and adjacent near-UV and near-infrared ranges. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied. Spectrophotometers were originally developed for the absorption measurement of liquid samples. However, development of high-precision, high-energy spectrophotometers allowed measurements in reflection and absorption on solid samples, including semiconductors, films, glass, and absorbing materials. UV-Vis spectroscopy is widely applied in the field of life sciences offering high-sensitivity and high-speed measurement of ultra-trace samples.

  • Wavelength range: 185 -1400nm
  • Photometric modes: Absorbance (Abs.), transmittance;(%), reflectance (%), energy (E)
  • Samples in liquid and solid states including smooth and rough surfaces

Photometric system: Double-beam optics
Photometric system: Czerny-Turner mounting: Single monochromator: Lo-Ray-Ligh grade blazed holographic grating
Detector: R-928 Photomultiplier
Light source: 50 W halogen lamp, deuterium lamp, Light source: auto position adjustment built in
Measurement wavelength range: 185 ~ 900nm: 220 nm to 1400 nm when the ISR-2600Plus: Integrating Sphere Attachment is used.
Wavelength accuracy: ±0.1 nm (656.1 nm D2), ±0.3 nm (all range)
Wavelength repeatability: ±0.05nm
Wavelength scanning speed: Wavelength slew rate: about 14000 m/min: Wavelength scan rate : about 4000 to 0.5 nm/min
Spectral bandwidth: 0.1/0.2/0.5/1/2/5 nm
Stray light ; Max. 0.005 % (220 nm, NaI); Max. 0.005 % (340, 370 nm, NaNO2); Max. 1 % (198 nm, KCl)
Photometric accuracy: ±0.002Abs (0.5Abs); ±0.003Abs (1Abs); ±0.006Abs (2Abs); ±0.3%T
Measurement wavelength range: ISR-2600 220 to 850 nm, ISR-2600Plus 220 to 1400nm

The data are taken from

SHIMADZU UV-VIS Spectrophotometer UV-2600Plus (with Integrating Sphere)


Transmittance Measurement



Diffuse Reflectance Measurement - ISR-2600Plus Integrating Sphere Attachment